DNA Transfection Screening from Single Beads
نویسندگان
چکیده
منابع مشابه
DNA transfection screening from single beads.
The solid-phase synthesis of a library of arginine-containing lipid transfection agents on high-loading beads is described. The transfection activity of the cationic lipids was determined using compound cleaved from single beads (single-bead screening) and showed, in some cases, comparable or higher DNA transfection activities as compared to commercially available reagents. Lipids with one argi...
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We have developed a novel method of genetic library construction on magnetic microbeads based on solid-phase single-molecule PCR in a fine and robust water-phase compartment formed in water-in-oil (w/o) emulsions. In this method, critically diluted DNA fragments were distributed over the emulsion as templates, where beads crosslinked with multiple primers and other PCR components were encapsula...
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BACKGROUND DNA promoter methylation is a signature for the silencing of tumor suppressor genes. Most widely used methods to detect DNA methylation involve 3 separate, independent processes: DNA extraction, bisulfite conversion, and methylation detection via a PCR method, such as methylation-specific PCR (MSP). This method includes many disconnected steps with associated losses of material, pote...
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Dye terminator C 1 #1 Taq polymerase 4 units 9. Prepare the individual reactions to contain 12/zl DNA template, 7.25/d reaction premix, and 1/zl primer (10 #M). 10. Cover with one drop of mineral oil and thermal cycle as follows: denature at 96 ° for 30 see, anneal at 50 ° for 15 see, and extend at 60 ° for 4 min. 11. Repeat for a total of 25 cycles. Note: The thermal cycle of this protocol is ...
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The plasma membrane of a eukaryotic cell is impermeable to most hydrophilic substances, yet the insertion of these materials into cells is an extremely important and universal requirement for the cell biologist. To address this need, many transfection techniques have been developed including viral, lipoplex, polyplex, capillary microinjection, gene gun and electroporation. The current discussio...
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ژورنال
عنوان ژورنال: Journal of Combinatorial Chemistry
سال: 2004
ISSN: 1520-4766,1520-4774
DOI: 10.1021/cc049945t